Journal: Cancer letters
Article Title: CD40 Drives Tumor Growth in Non-Small Cell Lung Cancer via non-canonical Immune-Independent Mechanisms
doi: 10.1016/j.canlet.2025.218151
Figure Lengend Snippet: A. Cell surface staining of CD40 protein in H23, PC-3, H1666, and H1648 NSCLC cells, after shRNA-mediated KD. B. Quantification of cell surface staining of CD40 protein in H23, PC-3, H1666, and H1648 NSCLC cells, after shRNA-mediated KD. C. Western blots of CD40 in H23, PC-3, H1666, and H1648 NSCLC cells after shRNA-mediated KD. D. Proliferation of H23, PC-3, H1666, and H1648 NSCLC cells following CD40 KD. PrestoBlue was used for cell proliferation measurements every day till 6 days post seeding. P value was determined by Friedman test. E. Bar graph showing growth of H23 xenograft tumor in Athymic nude mice. F. Bar graph showing growth of H23 xenograft in NGS mice G. Bar graph showing weight of tumors at endpoint in corresponding groups. P value was determined by two tailed paired t-test. H. Representative images of excised tumors from indicated groups.
Article Snippet: CD40 inhibitor DRI- C21045 (MedChemExpress) and CD40-TRAF6 inhibitor (Sigma, SML1160) were used as indicated.
Techniques: Knockdown, Staining, shRNA, Western Blot, Two Tailed Test