anti cd40  (Miltenyi Biotec)

Journal: Annals of Hematology

Article Title: Identification and validation of a prognostic risk-scoring model in drug-resistant ALL and evaluation of immune micro-environment

doi: 10.1007/s00277-026-07080-3

Figure Lengend Snippet: The CD40 agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant

Article Snippet: Phorbol 12-myristate 13-acetate (PMA, HY-18739), Daunorubicin hydrochloride (HY-13062), anti-mouse CD40 antibody (FGK45, HY-P990680) and anti-Human PD-L1 (HY-108730) were obtained from MedchemExpress.

Techniques: CCK-8 Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Injection, Control

Journal: Frontiers in Immunology

Article Title: Staphylococcus aureus serine protease-like protein B elicits a type 1/type 2 immune response in atopic dermatitis patients

doi: 10.3389/fimmu.2026.1798583

Figure Lengend Snippet: CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human CD40 and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.

Article Snippet: To prevent CD154 internalization and provide co-stimulation and a survival signal, agonistic antibodies against CD40 (1 μg/mL, 130-094-133, clone HB14, Miltenyi, Bergisch Gladbach, Germany) and CD28 (0.5 μg/mL, 555726, clone CD28.2, Becton Dickinson GmbH, Franklin Lakes, NJ, USA) were added for 24 h. To investigate SplB-specific T cells, PBMCs were stained with antibodies (unless otherwise stated: from BioLegend, San Diego, CA, USA): PerCP-conjugated anti-human CD4 (317432, clone OKT4) or PE-Cy5-conjugated anti-human CD4 (555348, clone RPA-T4, Becton Dickinson GmbH, Franklin Lakes, NJ, USA) BV510-conjugated anti-human CD14 (301842, clone M5E2), BV510-conjugated anti-human CD19 (302242; clone HIB19), FITC-conjugated anti-human CD154 (310804; clone 24-31), APC-conjugated anti-human CXCR3 (FAB160A, clone 49801, R&D Systems Inc, Minneapolis, MN, USA), BV421-conjugated anti-human CCR4 (359414, clone L291H4), PE-Cy7-conjugated anti-human CCR6 (353418, clone G034E3), and PE-conjugated anti-human CCR10 (130-120-407, clone: REA326, Miltenyi, Bergisch Gladbach, Germany) ( ).

Techniques: Isolation, Expressing, Control, MANN-WHITNEY, Comparison

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: TAC caused similar left ventricular hypertrophy, fibrosis and accumulation of immune cells in WT and CD40 KO mice. Post-TAC survival analysis of WT (n = 41) and CD40 KO (n = 37) mice (A). The ratios of LV and LA weight to tibial length of WT and CD40 KO mice under control or TAC condition (n = 9-13), Sham indicates no actual TAC (B, C). Representative images and summary data for LV myocyte cross-sectional area determined by FITC-conjugated wheat germ agglutinin (WGA) staining (n = 5-7) (D). Representative images and quantitative data of LV fibrosis by Sirius red/Fast green staining (n = 4-7) (E). CD45 immunostaining (red) and quantitative data of LV leukocyte infiltration (n = 5-6) (F). Survival rate was analyzed by Kaplan-Meier method and compared by log-rank test. All quantitative data are reported as means ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Control, Staining, Immunostaining

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: TAC caused comparable LV dysfunction in WT and CD40 KO mice. Representative echocardiograms of each group (A). Summary data for LV ejection fraction (EF%), fractional shortening (FS%), LV end-diastolic and end-systolic diameter (LV-EDD, LV-ESD) and heart rate (n = 9-13) in each group (B–F). LV pressure of WT (n = 5) and CD40 KO (n = 5) mice were measured at 8 weeks after TAC, representative invasive pressure curve(G) with quantifications of end-systolic pressure (ESP)(I) and end-diastolic pressure (EDP)(J). Representative dp/dt curve(H) with quantifications of dp/dtmax (maximal rate of change in systolic pressure over time) (L) and dp/dtmin (minimal rate of change in pressure over time) (M). The rate-pressure product (K) was calculated by multiplying the heart rate by the LV end-systolic pressure. Western blot of β-MHC and vinculin (loading control, n = 3-4) (N, O). All quantitative data are reported as mean ± SEM. The statistical significance was assessed using two-tailed Student's unpaired t tests(I-M). Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis (B–F, N, O). ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Western Blot, Control, Two Tailed Test

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO aggravated TAC-induced increase of lung weight. The ratios of lung wet weight, lung dry weight, lung water weight to tibial length (A-C). Lung water content percentage ((lung wet weight – lung dry weight)/lung wet weight × 100%) of each group mice (n = 7-13) (D). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques:

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO caused robust pulmonary microvascular thrombosis in mice after TAC. Representative images of hematoxylin and eosin (HE) staining of lung tissues from the experimental groups(A) . Representative immunostaining images of Carstairs staining of the lung (Fibrin: bright red, platelets: gray blue or navy, collagen: bright blue), CD42c, CD61 and vWF in the lung (B-E). Quantitative data of CD42c immunofluorescence staining in the lung (n = 5-7) (F). The platelet counts in each group of mice (n = 8-14) (G). Mean platelet volume (n = 8-14) (H). Quantitative data of vWF in the lung (n = 5-6) (I). The CD42c and vWF staining were expressed as a percentage of positive staining area to the total area. All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Staining, Immunostaining, Immunofluorescence

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO exacerbated platelet aggregation and blood clot retraction in mice after TAC. The aggregation levels of WT and CD40 KO mouse platelets in response to 1.2 μg/ml collagen stimulation (n = 3-5) (A). The aggregation levels of WT and CD40 KO mouse platelets in response to 0.067U/ml thrombin stimulation (n = 5-6) (B). The aggregation levels of WT and CD40 KO mouse platelets in response to 3.4U/ml ADP stimulation (n = 4-5) (C). Clot retraction of WT and CD40 KO mouse platelets (n = 5-7) (D). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques:

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO aggravated TAC-induced pulmonary leukocyte infiltration. Representative images and quantitative data of CD45 + cells, Mac2 + cells and CD3 + cells in lungs (n = 5-7) (A-C). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques:

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO exacerbated pulmonary fibrosis and vessel remodeling in mice after TAC. Representative images (A-C) and quantitative data (D-F) of Masson's trichrome staining and smooth muscle α-actin (red) of the lung tissues. Quantitative RT-PCR result of pulmonary TGFβ (normalized to 18S) (G). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Staining, Quantitative RT-PCR

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO exacerbated pulmonary oxidative stress in mice with existing LV dysfunction. Representative images and Quantitative data of pulmonary dihydroethidium (DHE) staining in mice (n = 5) (A, D), IHC analysis of 3′-nitrotyrosine (B, E) and 4-hydroxynonenal (n = 4-6) (C, F). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Staining

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: TAC resulted in significant RV inflammation, fibrosis and cardiomyocyte hypertrophy in CD40 KO mice. The ratio of RV weight to tibial length of WT and CD40 KO mice under control or TAC condition (n = 9-13) (A). Western blot of β-MHC and loading control of vinculin (n = 3-4) (B, C)). Representative images and summary data for RV myocyte cross-sectional area determined by FITC-conjugated WGA staining (n = 6) (D). Representative images and quantitative data of RV fibrosis by Sirius red/Fast green staining (n = 5-6) (E). CD45 immunostaining (red) and quantitative data of RV leukocyte infiltration (n = 5-7) (F). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Control, Western Blot, Staining, Immunostaining